Structural Polymorphism in Bacterial EspA Filaments Revealed by Cryo-EM and an Improved Approach to Helical Reconstruction
نویسندگان
چکیده
منابع مشابه
Structural polymorphism in bacterial EspA filaments revealed by cryo-EM and an improved approach to helical reconstruction.
The traditional Fourier-Bessel approach to three-dimensional reconstruction from electron microscopic (EM) images of helical polymers involves averaging over filaments, assuming a homogeneous structure and symmetry. We have used a real-space reconstruction approach to study the EspA filaments formed by enteropathogenic E. coli. In negative stain, the symmetry of these filaments is ambiguous, an...
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ژورنال
عنوان ژورنال: Structure
سال: 2006
ISSN: 0969-2126
DOI: 10.1016/j.str.2006.05.018